Phenotypic Characterization, Antimicrobial Susceptibility Patterns Profile and Risk Factors of Escherichia Colio157:H7 Isolated from Cattle Meat at Jimma Ethiopia
Eshetu Shumi1, *, Tadele Tolosa1, Mukarim Abdurahaman1, Abebe Olani2, Matios Lekew2,
Diriba Taddese
Jimma University College of Agriculture and Veterinary Medicine, Jimma, Oromia, Ethiopia
2National Animal Health Diagnostic and Investigation Center, Sebata, Oromia, Ethiopia
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*Corresponding author
To cite this article:
Eshetu Shumi, Tadele Tolosa, Mukarim Abdurahaman, Abebe Olani, Matios Lekew, Diriba Taddese. Phenotypic Characterization,
Antimicrobial Susceptibility Patterns Profile and Risk Factors of Escherichia Colio157:H7 Isolated from Cattle Meat at Jimma Ethiopia.
American Journal of Bioscience and Bioengineering. Vol. 9, No. 2, 2021, pp. 40-48. doi: 10.11648/j.bio.20210902.12
Abstract: Escherichia coli O157:H7 is considered the most prevalent food-borne pathogen that has gained increasing
attention worldwide in recent years. A cross-sectional study was undertaken from January 2018 to September 2018 on carcass
swabs and utensils at slaughterhouses as well as meat and utensils obtained from butcher shops at Jimma town to isolate identify
and assess association risk factors of Escherichia coli O157:H7 and its antimicrobial susceptibility patterns. From the total of 288
samples 51 of them were biochemically positive for Escherichia coli. From those isolated by biochemical tests four of them
were found to be positive for the Escherichia coli O157:H7 strain as confirmed by the Biolog incubation manual. Among the samples
(144) from the slaughterhouse examined 3 of the E. coli O157:H7 isolates were found to be positive for E. coli O157:H7. Two of
the isolates were identified from carcasses and one from hanging up wire at slaughter house while the rest one was from meat at
butcher shop. The antimicrobial susceptibility investigation of 4 E. coli O157:H7 isolates using 12 commonly marketable
antimicrobial discs revealed that the isolates were susceptible to eight antimicrobials from 75% to 100%. Results of the present
study indicated that an overall resistance of 100%, 75%, 50% and 25% was observed to Ampicillin, Neomycin, Cefoxitin and
Oxytetracycline respectively. Multi-drug resistance to three drugs was detected in 1 (25%) of the isolates. This study indicates
the possible risk of E. coli O157:H7 particularly for the consumers who have the habit of eating raw or undercooked meat.
These findings stress the need for implementation of E. coli O157:H7 prevention and control strategies from slaughterhouse to
butcher shop and consumption of meat.
Read more: https://www.sciencepublishinggroup.com/article/10.11648/j.bio.20210902.12